In the light of day and in the dead of night, mysterious horrors await in the darkest shadows of every corner. They are unexplainable, inescapable, and undefeatable. Be prepared, or you may become their next victim. Sit back in terror as traumatizing tales of unparalleled terror unfold. Tales, such as that of a cursed jade carving that opens holes all over its victims' bodies; deep nightmares that span decades; an attractive spirit at a misty crossroad that grants cursed advice; and a slug that grows inside a girl's mouth. Tread carefully, for the horrifying supernatural tales of the Itou Junji: Collection are not for the faint of heart.

Keima Katsuragi, known online as the legendary "God of Conquest," can conquer any girl's heart—in dating sim games, at least. In reality, he opts for the two-dimensional world of gaming over real life because he is an unhealthily obsessed otaku of galge games (a type of Japanese video game centered on interactions with attractive girls). When he arrogantly accepts an anonymous offer to prove his supremacy at dating sim games, Keima is misled into aiding a naïve and impish demon from hell named Elucia "Elsie" de Lute Ima with her mission: retrieving runaway evil spirits who have escaped from hell and scattered themselves throughout the human world. Keima discovers that the only way to capture these spirits is to conquer what he hates the most: the unpredictable hearts of three-dimensional girls! Shackled to Elsie via a deadly collar, Keima now has his title of "God of Conquest" put to the ultimate test as he is forced to navigate through the hearts of a multitude of real-life girls. [Written by MAL Rewrite]

The world as we know it is actually just half the story, as Takeshi Nanase finds out abruptly one summer morning. On his way to kendo practice, Takeshi comes across an unconscious girl in a uniform he doesn't recognize. Takeshi does the decent thing and saves her, and in return the girl wakes up and accidentally turns him into a magic-user. As Takeshi finds out, there is the world he lives in and the world of magic users. Most magic users just want to peacefully coexist with non-magicians, but there are some with bigger ambitions. Mui Aiba is a magician enrolled in the Subaru Magic Academy, where magic users can learn to control and channel their powers and how to live in peace with regular humans. After his fateful encounter with Mui, Takeshi and his newly magician friends Kurumi Isoshima and Kazumi Ida decide to enroll in the Magic Academy as well. All three friends have different reasons for fighting on, whether they're fighting to escape the past or catch up to the future. They wield different kinds of powers, which they must learn to harness in order to fight off the Ghost Trailers, a group of magicians who are willing to use violence to assert their superiority over humans. Pursued by the Ghost Trailers, Takeshi and his friends must train to become stronger, face the leader of the Trailers, and prevent the beginning of the Second Great Magic War.

Dwelling in the small outskirts of a peaceful town, Lawrence works as a local pastor for the townsfolk. When in need, he is responsible for guiding people to the church to visit the "Saint"—a symbol of good morals who heeds each parishioner's struggles and grants them sincere advice. Cecilia, a kind young girl, is tasked to fulfill this role; however, this tiresome job quickly exhausts her. That, paired with her hidden somnolent nature, often causes her to laze around the church. This leaves Lawrence to care for the church all by himself. While Cecilia sleeps in anticipation for her next visitor, Lawrence caters to her needs alongside performing the church's numerous responsibilities. Slowly but surely, they begin to form a comfortable and trusting friendship with one another. Lawrence accepting Cecilia's lax nature elates her while she grows accustomed to Lawrence's overly-doting nature. However, as the adoring and absent-minded saint's feelings for the dense and overly protective pastor progress past friendship, how will he respond?